Tag

Part:BBa_K3568007

Designed by: MENG XU   Group: iGEM20_XH-China   (2020-10-21)


polyhistidine tag

Permits purification of recombinant fusion protein on metal-chelating resins. A his-tag, is a string of histidine residues at either the N or C terminus of a recombinant protein. His-tag purification uses the purification technique of immobilized metal affinity chromatography, or IMAC. The his-tag has a high affinity for these metal ions and binds strongly to the IMAC column. The use of a his-tag and IMAC can often provide relatively pure recombinant protein directly from a crude lysate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None